[Distinctive features of the self-association of deoxyhexanucleotide 5'-D(GpCpApTpGpC) in aqueous solution: 1H NMR analysis]. / Osobennosti protsessa samoassotsiatsii molecul dezoksigeksanukleotida 5'-D(GpCpApTpGpC) v vodnom rastvore: 1H-IAMR-analiz.
Biofizika
; 49(5): 781-8, 2004.
Article
en Ru
| MEDLINE
| ID: mdl-15526461
The self-association of self-complementary deoxyhexanucleotide d(GCATGC) was investigated in aqueous salt solution. Homonuclear 1H NMR correlation spectroscopy (2D-TOCSY and 2D-NOESY) was used for complete assignments of nonexchangeable protons of the hexamer. The equilibrium reaction constants and thermodynamical parameters of duplex d(GCATGC)2 formation were determined from experimental concentration and temperature dependences of proton chemical shifts of the deoxyhexanucleotide. Distinctive features of the concentration dependences in the range of small concentrations at relatively low temperatures of solution enable one to assume that one single-stranded hexamer sequence forms a compact structure (similar to a hairpin) in aqueous solution. A possible spatial hairpin structure of the hexamer was proposed. Comparative analysis of the experimental and theoretical (using the "nearest neighbor" model) thermodynamical parameters of duplex formation was made.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligodesoxirribonucleótidos
/
ADN
/
Agua
Tipo de estudio:
Risk_factors_studies
Idioma:
Ru
Revista:
Biofizika
Año:
2004
Tipo del documento:
Article
Pais de publicación:
Rusia