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Heavy metal accumulation, heat shock protein expression and cytogenetic changes in Tetrix tenuicornis (L.) (Tetrigidae, Orthoptera) from polluted areas.
Warchalowska-Sliwa, E; Niklinska, M; Görlich, A; Michailova, P; Pyza, E.
Afiliación
  • Warchalowska-Sliwa E; Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Slawkowska 17, 31-016 Kraków, Poland. warchalowska@isez.pan.krakow.pl
Environ Pollut ; 133(2): 373-81, 2005 Jan.
Article en En | MEDLINE | ID: mdl-15519468
The orthopteran insect Tetrix tenuicornis, collected from polluted and unpolluted areas, was used to study heavy metal accumulation and its impact on stress protein levels and on changes in the number and morphology of chromosomes in mitotic and meiotic cells. During two consecutive years, insects were collected from polluted areas of zinc-lead mine spoils near Boleslaw (Poland) and from unpolluted areas near Busko and Staszów (Poland). T. tenuicornis from the polluted area showed 1.5, 4.03, 4.32 and 41.73 times higher concentrations of copper (Cu), zinc (Zn), lead (Pb) and cadmium (Cd), respectively, than insects of the same species collected from unpolluted areas. Insects exposed to heavy metals showed only small changes, and rather a decrease in the concentration of constitutive and inducible heat shock proteins Hsp70, the level of which increases under stress conditions. A cytogenetic study of T. tenuicornis revealed intra-population anomalies in chromosome number and morphology in mitotic and meiotic cells and the presence of an additional B chromosome in germinal cells. In 50% of females collected from polluted areas, mosaic oogonial mitotic chromosome sets and diploid, hypo- or hypertetraploid, tetraploid, and octoploid chromosome numbers were detected. In turn, 14.6% of males showed a heterozygous deficiency of chromatin in L2 and M3 bivalents in addition to the presence of B chromosomes.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ortópteros / Metales Pesados / Contaminantes Ambientales / Proteínas de Choque Térmico Límite: Animals Idioma: En Revista: Environ Pollut Asunto de la revista: SAUDE AMBIENTAL Año: 2005 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ortópteros / Metales Pesados / Contaminantes Ambientales / Proteínas de Choque Térmico Límite: Animals Idioma: En Revista: Environ Pollut Asunto de la revista: SAUDE AMBIENTAL Año: 2005 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido