[Modification and application of recombinant yeast bioassay for measuring the AhR ligand activity].
Huan Jing Ke Xue
; 25(4): 155-8, 2004 Jul.
Article
en Zh
| MEDLINE
| ID: mdl-15515957
A recombinant yeast bioassay, a yeast Saccharomyces cerevisiae, in which the human AhR and ARNT complex are coexpressed, is one of the methods to screen the active AhR agonist. In this study, the original agonist test was modified. The exposure time was reduced from 18 to 8 hours when experiment was under the following conditions: (1) the yeast was cultured in 0.2% glucose medium for 24 hours; (2) chemical exposure was carried in 2% galactose medium in glass tube. Finally, the AhR acitivity of hexachlorobenzene and pentachlorobenzene were assessed, and their toxicity equivalent factors were found to be 0.018629 and 0.000294, respectively.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Receptores de Hidrocarburo de Aril
/
Translocador Nuclear del Receptor de Aril Hidrocarburo
Límite:
Humans
Idioma:
Zh
Revista:
Huan Jing Ke Xue
Año:
2004
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China