Estimate of the sensitivity of an ELISA used to detect Johne's disease in Victorian dairy cattle herds.
Aust Vet J
; 82(9): 569-73, 2004 Sep.
Article
en En
| MEDLINE
| ID: mdl-15478730
OBJECTIVE: To estimate the sensitivity of the ELISA used in dairy cattle herds participating in the Victorian Bovine Johne's Disease Test and Control Program (TCP). PROCEDURE: The percentage of ELISA reactors in age and test cohorts was estimated from age-specific test data derived from TCP herds with long testing histories. Age-distribution data from production-tested herds enabled estimation of reactor rates in animals that were culled or died. RESULTS: ELISA sensitivities at the first test round in herds achieving five, six and seven annual herd tests were 16.1, 14.9 and 13.5% respectively. The ELISA sensitivity in 2, 3 and 4-year-old animals at the first test round in herds testing seven times was 1.2, 8.9 and 11.6% respectively but remained between 20 and 30% in older age-groups. CONCLUSION: The sensitivity of the ELISA is considerably lower than previous estimates, probably because previous estimates were predominantly measured against faecal culture, which has subsequently been shown to have low sensitivity itself, and did not appreciate the long period that appears to precede detectable faecal excretion in most animals.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Paratuberculosis
/
Ensayo de Inmunoadsorción Enzimática
/
Enfermedades de los Bovinos
Tipo de estudio:
Diagnostic_studies
/
Etiology_studies
/
Evaluation_studies
/
Prognostic_studies
Aspecto:
Patient_preference
Límite:
Animals
País/Región como asunto:
Oceania
Idioma:
En
Revista:
Aust Vet J
Año:
2004
Tipo del documento:
Article
Pais de publicación:
Reino Unido