Chlorine transfer between glycine, taurine, and histamine: reaction rates and impact on cellular reactivity.

Peskin, Alexander V; Midwinter, Robyn G; Harwood, David T; Winterbourn, Christine C

Peskin, Alexander V; Midwinter, Robyn G; Harwood, David T; Winterbourn, Christine C.

Afiliación

Peskin AV; Free Radical Research, Department of Pathology, Christchurch School of Medicine and Health Sciences, P.O. Box 4345, Christchurch, New Zealand. alexander.peskin@chmeds.ac.nz

Free Radic Biol Med ; 37(10): 1622-30, 2004 Nov 15.

Article en En | MEDLINE | ID: mdl-15477013

RESUMEN

Hypochlorous acid formed by activated neutrophils reacts with amines to produce chloramines. Chloramines vary in stability, reactivity, and cell permeability. We have examined whether chloramine exchange occurs between physiologically important amines or amino acids and if this affects interactions of chloramines with cells. We have demonstrated transchlorination reactions between histamine, glycine, and taurine chloramines by measuring chloramine decay rates with mixtures as well as by mass spectrometry. Kinetic analysis suggested the formation of an intermediate complex with a high K(m). Apparent second-order rate constants, determined for concentrations

Asunto(s)

Cloraminas/química; Cloro/química; Glicina/química; Histamina/química; Taurina/química; Células Cultivadas; Células Endoteliales/química; Células Endoteliales/citología; Gliceraldehído-3-Fosfato Deshidrogenasas/química; Humanos; Ácido Hipocloroso/química; Células Jurkat

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Taurina / Histamina / Cloraminas / Cloro / Glicina Límite: Humans Idioma: En Revista: Free Radic Biol Med Asunto de la revista: BIOQUIMICA / MEDICINA Año: 2004 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Estados Unidos

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links