Heme oxygenase exerts a protective role against oxidative stress in soybean leaves.
Biochem Biophys Res Commun
; 323(3): 1003-8, 2004 Oct 22.
Article
en En
| MEDLINE
| ID: mdl-15381099
We have previously demonstrated that the induction of heme oxygenase-1 (EC 1.14.99.3) plays a protective role for mammalian cells against oxidative stress. Here, we investigated for the first time the possible role of heme oxygenase-1 as an antioxidant defense in leaves of soybean plants. Treatment with 200 microM Cd during 48 h caused a 70% increase in thiobarbituric acid reactive substances, whereas GSH decreased 67%, guaiacol peroxidase and total superoxide dismutase also inhibited 49% and 46%, respectively. Two hundred micromolar of Cd produced the overexpression of heme oxygenase-1, as well as a 4.5-fold enhancement of its activity. Administration of biliverdin partially prevented the effects caused by Cd. Pretreatment with Zn protoporphyrin IX, a potent inhibitor of heme oxygenase, expectedly decreased heme oxygenase-1 activity to half. When the inhibitor was given before Cd, it completely prevented the enzyme induction increasing the levels of oxidative stress parameters. Collectively, these results indicated that although plant heme oxygenases share little homology to heme oxygenases from non-plant species, they also play an important protective role against oxidative cell damage.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glycine max
/
Especies Reactivas de Oxígeno
/
Hojas de la Planta
/
Estrés Oxidativo
/
Hemo Oxigenasa (Desciclizante)
/
Antioxidantes
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2004
Tipo del documento:
Article
País de afiliación:
Argentina
Pais de publicación:
Estados Unidos