Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines.

Wang, P; Mariman, E; Keijer, J; Bouwman, F; Noben, J-P; Robben, J; Renes, J

Wang, P; Mariman, E; Keijer, J; Bouwman, F; Noben, J-P; Robben, J; Renes, J.

Afiliación

Wang P; Maastricht Proteomics Center, The Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Department of Human Biology, Maastricht University, 6200 MD Maastricht, The Netherlands.

Cell Mol Life Sci ; 61(18): 2405-17, 2004 Sep.

Article en En | MEDLINE | ID: mdl-15378209

RESUMEN

Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20 degrees C treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation.

Asunto(s)

Adipocitos/citología; Adipocitos/metabolismo; Diferenciación Celular; Proteínas/análisis; Proteínas/metabolismo; Células 3T3-L1; Adipocitos/efectos de los fármacos; Secuencia de Aminoácidos; Animales; Biomarcadores/análisis; Brefeldino A/farmacología; Electroforesis en Gel Bidimensional; Matriz Extracelular/metabolismo; Sustancias de Crecimiento/análisis; Sustancias de Crecimiento/química; Sustancias de Crecimiento/metabolismo; Ratones; Datos de Secuencia Molecular; Proteínas/química; Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción; Temperatura; Regulación hacia Arriba

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Diferenciación Celular / Adipocitos Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Cell Mol Life Sci Asunto de la revista: BIOLOGIA MOLECULAR Año: 2004 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Suiza

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