Predominant expression of GluR2 among the AMPA receptor subunits in neuronal progenitor cells of the rat hippocampus.
Brain Res Dev Brain Res
; 152(2): 213-23, 2004 Sep 17.
Article
en En
| MEDLINE
| ID: mdl-15351509
Cells derived from the hippocampus of embryonic day 18 (E18) rats were cultured in B27-supplemented Neurobasal medium without serum. We found the presence of numerous small cells with round or elliptical somata and fine processes in this primary culture. These cells were first detectable on culture day 8 and gradually increased in number that reached the maximum on approximately day 14. They incorporated bromodeoxyuridine (BrdU) and expressed nestin, a marker of stem cells and progenitor cells. Furthermore, nearly a half of these cells also expressed neuron-specific beta tubulin. On the other hand, they did not express O4 and glial fibrillary acid protein (GFAP), markers of oligodendrocytes and astrocytes, respectively. Thus, these small cells are most likely to be neuronal progenitor cells. The whole-cell patch clamp studies revealed that these cells expressed voltage-gated Na+, Ca2+ and K+ channels. With regard to ligand-gated channels, these cells were sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA), but not to N-methyl-D-aspartate (NMDA). The current-voltage relationship of the AMPA-induced current was slightly outwardly rectifying, suggesting that the AMPA receptors contained the GluR2 subunit in their oligomeric assemblies. The single-cell reverse transcription (RT)-PCR analysis revealed that GluR2 is predominant over the other AMPA receptor subunits in these cells. Furthermore, GluR2 was expressed mainly in the flip form.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Receptores AMPA
/
Hipocampo
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Brain Res Dev Brain Res
Asunto de la revista:
CEREBRO
/
NEUROLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Países Bajos