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Styrene 7,8-oxide induces mitochondrial damage and oxidative stress in neurons.
Daré, E; Tofighi, R; Nutt, L; Vettori, M V; Emgård, M; Mutti, A; Ceccatelli, S.
Afiliación
  • Daré E; Institute of Environmental Medicine, Division of Toxicology and Neurotoxicology, Karolinska Institutet, Box 210, S-171 77 Stockholm, Sweden. elisabetta.dare@imm.ki.se
Toxicology ; 201(1-3): 125-32, 2004 Sep 01.
Article en En | MEDLINE | ID: mdl-15297027
Styrene 7,8-oxide (SO) is the main metabolite of styrene, a neurotoxic compound used industrially. Neurons exposed to SO undergo apoptosis with characteristic features including chromatin rearrangements and caspase activation. We report that the execution phase of apoptosis induced by SO (0.3 mM) in SK-N-MC neurons is triggered by translocation of apoptogenic factors (e.g., cytochrome c) into the cytosol. In addition, mitochondria exhibit lower Ca2+ capacity and loss of mitochondrial membrane potential (DeltaPsi). Lipid peroxidation, measured as thiobarbituric acid reactive substances (TBARS), is increased after 12 h. Pre-treatment with the antioxidant MnTBAP (100 microM) prevents the decrease of Ca2+ capacity, cytochrome c release, activation of caspases, exposure of phosphatidylserine and cell death. Hence, the neurotoxic effects of SO are related to mitochondrial damage and oxidative stress.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinógenos / Calcio / Apoptosis / Estrés Oxidativo / Citocromos c / Compuestos Epoxi / Mitocondrias / Neuronas Límite: Humans Idioma: En Revista: Toxicology Año: 2004 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Irlanda
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carcinógenos / Calcio / Apoptosis / Estrés Oxidativo / Citocromos c / Compuestos Epoxi / Mitocondrias / Neuronas Límite: Humans Idioma: En Revista: Toxicology Año: 2004 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Irlanda