Synthesis of flavor and fragrance esters using Candida antarctica lipase.
Appl Microbiol Biotechnol
; 65(4): 373-6, 2004 Sep.
Article
en En
| MEDLINE
| ID: mdl-15248036
Candida antarctica lipase fraction B (CAL-B) showed substrate specificity in the synthesis of esters in hexane involving reactions of short-chain acids having linear (acetic and butyric acids) and branched chain (isovaleric acid) structures, an unsaturated (tiglic acid) fatty acid, and phenylacetic acid with n-butanol and geraniol. The variation in the conversion to the esters was ca. 10%. Similar results were observed in a study of the alcohol specificity of the enzyme for esterification of acetic and butyric acids with four alcohols: n-butyl, isopentyl, 2-phenylethyl, and geraniol. Enantioselectivity of CAL-B in hexane with a range of chiral alpha-substituted or beta-substituted carboxylic acids and n-butyl alcohol was analyzed. The results show that CAL-B can be employed as a robust biocatalyst in esterification reactions due to the high conversions obtained in the synthesis of short-chain flavor esters in an organic solvent, although this enzyme exhibited modest enantioselectivity with chiral short-chain carboxylic acids.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Perfumes
/
Biotecnología
/
Candida
/
Ésteres
/
Aromatizantes
/
Lipasa
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
2004
Tipo del documento:
Article
País de afiliación:
México
Pais de publicación:
Alemania