Isotachophoresis of beta-blockers in a capillary and on a poly(methyl methacrylate) chip.
Electrophoresis
; 25(10-11): 1687-94, 2004 Jun.
Article
en En
| MEDLINE
| ID: mdl-15188258
Analysis of the beta-blockers oxprenolol, atenolol, timolol, propranolol, metoprolol, and acebutolol in human urine by a combination of isotachophoresis (ITP) and zone electrophoresis (ZE) was investigated. Methods were developed with a conventional capillary electrophoresis (CE) apparatus and a poly(methyl methacrylate) (PMMA) microchip system. With CE the separation of oxprenolol, atenolol, timolol, and acebutolol from a standard solution containing 5 microg/mL of each compound was accomplished by performing ZE with transient ITP. The electrolyte system consisted of 10 mM sodium morpholinoethane sulfonate (pH 5.5) and 0.1% methylhydroxyethylcellulose as the leading electrolyte and 30 mM ortho-phosphoric acid (pH 2.0) as both the terminating and the ZE background electrolyte. With the microchip system the separation of oxprenolol and acebutolol from a standard solution containing 10 microg/mL of each compound was accomplished by a coupled-channel ITP-ZE device using the same leading electrolyte solution as the CE system but 5 mM glutamic acid (pH 3.4) as terminating and background electrolytes. The systems were used for analyses of patient urine samples. Water-soluble hydrophilic matrix compounds were removed from the urine samples by solid-phase extraction (SPE). Limits of quantification below 5 microg/mL could be achieved. The PMMA ITP-ZE chip has not earlier been used for analyses of any drugs from urine samples.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antagonistas Adrenérgicos beta
/
Electroforesis Capilar
/
Electroforesis
/
Microquímica
Límite:
Humans
Idioma:
En
Revista:
Electrophoresis
Año:
2004
Tipo del documento:
Article
País de afiliación:
Finlandia
Pais de publicación:
Alemania