Identification of nuclear tri-iodothyronine receptors in Sertoli cells from immature piglet testes.

Palmero, S; Benahmed, M; Morera, A M; Trucchi, P; Fugassa, E

Palmero, S; Benahmed, M; Morera, A M; Trucchi, P; Fugassa, E.

Afiliación

Palmero S; Istituto di Fisiologia Generale, Università degli Studi di Genova, Italy.

J Mol Endocrinol ; 9(1): 55-9, 1992 Aug.

Article en En | MEDLINE | ID: mdl-1515025

RESUMEN

The existence of nuclear tri-iodothyronine (T3) receptors in both Sertoli and Leydig cells isolated from immature piglet testes was investigated. The results demonstrated the presence of high-affinity (Kd = 1.09 +/- 0.25 nM), low-capacity (185 +/- 24 pg T3/mg DNA) binding sites for T3 in nuclei from freshly isolated Sertoli cells. No specific binding for T3 was observed in nuclei isolated from Leydig cells. The localization of specific T3 receptors, which might mediate the onset of thyroid hormone action, at Sertoli cell level confirms that these cells are a target for thyroid hormone and strongly sustain the role of the thyroid in the regulation of testicular functions during postnatal development.

Asunto(s)

Núcleo Celular/metabolismo; Receptores de Hormona Tiroidea/metabolismo; Células de Sertoli/metabolismo; Testículo/metabolismo; Animales; Sitios de Unión; Células Cultivadas; Células Intersticiales del Testículo/metabolismo; Masculino; Porcinos; Testículo/citología

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de Sertoli / Testículo / Receptores de Hormona Tiroidea / Núcleo Celular Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Mol Endocrinol Asunto de la revista: BIOLOGIA MOLECULAR / ENDOCRINOLOGIA Año: 1992 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido

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