Your browser doesn't support javascript.
loading
Molecular typing of Pneumocystis jirovecii found in formalin-fixed paraffin-embedded lung tissue sections from sudden infant death victims.
Chabé, M; Vargas, S L; Eyzaguirre, I; Aliouat, E M; Follet-Dumoulin, A; Creusy, C; Fleurisse, L; Recourt, C; Camus, D; Dei-Cas, E; Durand-Joly, I.
Afiliación
  • Chabé M; Ecology of Parasitism, EA-3609-IFR17, Pasteur Institute of Lille, 1 rue du Professeur-Calmette BP245, 59019 Lille, France.
  • Vargas SL; Respiratory Infections Laboratory, Biomedical Sciences Institute, University of Chile School of Medicine, Independencia 1027, Santiago, Chile.
  • Eyzaguirre I; Respiratory Infections Laboratory, Biomedical Sciences Institute, University of Chile School of Medicine, Independencia 1027, Santiago, Chile.
  • Aliouat EM; Ecology of Parasitism, EA-3609-IFR17, Pasteur Institute of Lille, 1 rue du Professeur-Calmette BP245, 59019 Lille, France.
  • Follet-Dumoulin A; Ecology of Parasitism, EA-3609-IFR17, Pasteur Institute of Lille, 1 rue du Professeur-Calmette BP245, 59019 Lille, France.
  • Creusy C; Free Faculty of Medicine, Lille Catholic University, rue du Port, 59046 Lille, France.
  • Fleurisse L; Free Faculty of Medicine, Lille Catholic University, rue du Port, 59046 Lille, France.
  • Recourt C; Ecology of Parasitism, EA-3609-IFR17, Pasteur Institute of Lille, 1 rue du Professeur-Calmette BP245, 59019 Lille, France.
  • Camus D; Parasitology-Mycology Service, Faculty of Medicine, Lille-2 University Hospital Center, 1 place Verdun, 59045 Lille, France.
  • Dei-Cas E; Ecology of Parasitism, EA-3609-IFR17, Pasteur Institute of Lille, 1 rue du Professeur-Calmette BP245, 59019 Lille, France.
  • Durand-Joly I; Parasitology-Mycology Service, Faculty of Medicine, Lille-2 University Hospital Center, 1 place Verdun, 59045 Lille, France.
Microbiology (Reading) ; 150(Pt 5): 1167-1172, 2004 May.
Article en En | MEDLINE | ID: mdl-15133076
Previous studies have provided histological evidence of an association between primary Pneumocystis infection and sudden infant death syndrome (SIDS). The aim of this work was to determine the species of clustered Pneumocystis organisms found in formalin-fixed paraffin-embedded (FFPE) lung tissue sections from Chilean sudden infant death (SID) victims. This approach needed first to optimize a DNA extraction method from such histological sections. For that purpose, the QIAamp DNA Isolation from Paraffin-Embedded Tissue method (Qiagen) was first tested on FFPE lung tissue sections of immunosuppressed Wistar rats inoculated with rat-derived PNEUMOCYSTIS: Successful DNA extraction was assessed by the amplification of a 346 bp fragment of the mitochondrial large subunit rRNA gene of the Pneumocystis species using a previously described PCR assay. PCR products were analysed by direct sequencing and sequences corresponding to Pneumocystis carinii were found in all the samples. This method was then applied to FFPE lung tissue sections from Chilean SID victims. Pneumocystis jirovecii was successfully identified in the three tested samples. In conclusion, an efficient protocol for isolating PCR-ready DNA from FFPE lung tissue sections was developed. It established that the Pneumocystis species found in the lungs of Chilean SID victims was P. jirovecii.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía por Pneumocystis / Muerte Súbita del Lactante / Adhesión en Parafina / Pneumocystis carinii / Formaldehído / Pulmón Límite: Animals / Female / Humans / Infant País/Región como asunto: America do sul / Chile Idioma: En Revista: Microbiology (Reading) Asunto de la revista: MICROBIOLOGIA Año: 2004 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía por Pneumocystis / Muerte Súbita del Lactante / Adhesión en Parafina / Pneumocystis carinii / Formaldehído / Pulmón Límite: Animals / Female / Humans / Infant País/Región como asunto: America do sul / Chile Idioma: En Revista: Microbiology (Reading) Asunto de la revista: MICROBIOLOGIA Año: 2004 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido