Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter.
Lab Chip
; 3(1): 22-7, 2003 Feb.
Article
en En
| MEDLINE
| ID: mdl-15100801
The integration of complete analyses systems "on chip" is one of the great potentials of microfabricated devices. In this study we present a new pressure-driven microfabricated fluorescent-activated cell sorter chip with advanced functional integration. Using this sorter, fluorescent latex beads are sorted from chicken red blood cells, achieving substantial enrichments at a sample throughput of 12000 cells s(-1). As a part of the sorter chip, we have developed a monolithically integrated single step coaxial flow compound for hydrodynamic focusing of samples in flow cytometry and cell sorting. The structure is simple, and can easily be microfabricated and integrated with other microfluidic components. We have designed an integrated chamber on the chip for holding and culturing of the sorted cells. By integrating this chamber, the risk of losing cells during cell handling processes is eliminated. Furthermore, we have also developed integrated optics for cell detection. Our new design contributes to the ongoing efforts for building a fully integrated micro cell sorting and analysing system.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microfluídica
/
Eritrocitos
/
Citometría de Flujo
Límite:
Animals
Idioma:
En
Revista:
Lab Chip
Asunto de la revista:
BIOTECNOLOGIA
/
QUIMICA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Dinamarca
Pais de publicación:
Reino Unido