Determination of strophanthidin in ingesta and plant material by LC-MS/MS.
J Agric Food Chem
; 52(8): 2174-8, 2004 Apr 21.
Article
en En
| MEDLINE
| ID: mdl-15080617
An LC-MS/MS method was developed for the semiquantitative determination of strophanthidin glycosides in ingesta from animals. Strophanthidin glycosides were simultaneously extracted and hydrolyzed to the strophanthidin aglycone using aqueous methanolic hydrochloric acid and the extracts cleaned up using solid-phase extraction. Extracts were analyzed using reverse-phase HPLC coupled with positive ion electrospray mass spectrometry. Characteristic product ion spectra were produced by fragmentation of the [M + H](+) precursor ion for each analyte. Quantitation was performed using the internal standard method with digitoxigenin serving as the internal standard. The method detection limit was calculated to be 0.075 microg/g, and the limit of quantitation was calculated to be 0.24 microg/g for strophanthidin in control rumen samples. This method was used in diagnostic investigations to confirm fatal strophanthidin glycoside poisonings in horses.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plantas
/
Estrofantidina
/
Cromatografía Líquida de Alta Presión
/
Espectrometría de Masa por Ionización de Electrospray
/
Sistema Digestivo
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Agric Food Chem
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos