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Sublocalization of an invasion-inducing locus and other genes on human chromosome 7.
Habets, G G; van der Kammen, R A; Willemsen, V; Balemans, M; Wiegant, J; Collard, J G.
Afiliación
  • Habets GG; The Netherlands Cancer Institute, Division of Cell Biology, Amsterdam.
Cytogenet Cell Genet ; 60(3-4): 200-5, 1992.
Article en En | MEDLINE | ID: mdl-1505215
By somatic cell fusion studies between noninvasive mouse T-lymphoma cells and invasive human activated normal T-cells we have previously shown that the genetic information responsible for the induction of invasive and metastatic potential in interspecies T-cell hybrids is located on human chromosome 7. Apparently, genes derived from normal activated T-cells are dominantly expressed in the hybrids and control the invasive and, as a consequence, metastatic potential of these T-lymphoma cells. To sublocalize the invasion-inducing locus on chromosome 7 we have generated hybrids that harbor only specific regions of human chromosome 7 with or without a small fragment of human chromosome 21. Analysis of these hybrids revealed that the invasion-inducing locus maps to 7p12----cen. The human DNA complement of the hybrids was confirmed by Southern blot analysis using a large panel of chromosome 7-specific DNA probes. Several of these genes could be further sublocalized. These included: ARAF2 to 7p12----cen, D7S21 to 7pter----p12, ACTB to 7p15----p12, EGFR to 7p12, MDH2 to 7cen----q22, and PDGFA to 7pter----p15.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosomas Humanos Par 7 / Invasividad Neoplásica Límite: Humans Idioma: En Revista: Cytogenet Cell Genet Año: 1992 Tipo del documento: Article Pais de publicación: Suiza
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosomas Humanos Par 7 / Invasividad Neoplásica Límite: Humans Idioma: En Revista: Cytogenet Cell Genet Año: 1992 Tipo del documento: Article Pais de publicación: Suiza