Effect of 50 Hz electromagnetic fields on the induction of heat-shock protein gene expression in human leukocytes.
Radiat Res
; 161(4): 430-4, 2004 Apr.
Article
en En
| MEDLINE
| ID: mdl-15038769
Although evidence is controversial, exposure to environmental power-frequency magnetic fields is of public concern. Cells respond to some abnormal physiological conditions by producing cytoprotective heat-shock (or stress) proteins. In this study, we determined whether exposure to power-frequency magnetic fields in the range 0-100 microT rms either alone or concomitant with mild heating induced heat-shock protein gene expression in human leukocytes, and we compared this response to that induced by heat alone. Samples of human peripheral blood were simultaneously exposed to a range of magnetic-field amplitudes using a regimen that was designed to allow field effects to be distinguished from possible artifacts due to the position of the samples in the exposure system. Power-frequency magnetic-field exposure for 4 h at 37 degrees C had no detectable effect on expression of the genes encoding HSP27, HSP70A or HSP70B, as determined using reverse transcriptase-PCR, whereas 2 h at 42 degrees C elicited 10-, 5- and 12-fold increases, respectively, in the expression of these genes. Gene expression in cells exposed to power-frequency magnetic fields at 40 degrees C was not increased compared to cells incubated at 40 degrees C without field exposure. These findings and the extant literature suggest that power-frequency electromagnetic fields are not a universal stressor, in contrast to physical agents such as heat.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Campos Electromagnéticos
/
Proteínas de Choque Térmico
/
Leucocitos
Límite:
Adult
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Radiat Res
Año:
2004
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
Estados Unidos