A molecular marker for the identification of Simulium squamosum (Diptera: Simuliidae).
Ann Trop Med Parasitol
; 98(2): 197-208, 2004 Mar.
Article
en En
| MEDLINE
| ID: mdl-15035730
All except one of the important groups of West African vectors of Onchocerca volvulus that lie within the Simulium damnosum complex can be distinguished from each other using morphological characteristics. The exception is S. squamosum, which overlaps with other species, and this results in significant levels of misidentification. Variation in the untranscribed H3-H4 histone intergenic spacer region of flies of the S. damnosum complex has now been investigated. Although a CAA/CAG microsatellite was found to be hyper-variable and unsuitable for species diagnosis, a 10-bp indel seemed to vary in a species-specific manner. This indel was apparently absent from S. squamosum but present in all of the other species tested (S. damnosum s.s., S. sirbanum, S yahense, S. sanctipauli and S. leonense/konkourense). It should now be possible to identify individual, adult, female S. squamosum from the absence of the indel, using a PCR-based amplification and agarose- or polyacrylamide-gel electrophoresis, thus removing the major barrier to the routine identification of unknown samples.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Simuliidae
/
Insectos Vectores
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
País/Región como asunto:
Africa
Idioma:
En
Revista:
Ann Trop Med Parasitol
Año:
2004
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Reino Unido