Lipoprotein lipase and endothelial lipase expression in mouse brain: regional distribution and selective induction following kainic acid-induced lesion and focal cerebral ischemia.

Paradis, E; Clavel, S; Julien, P; Murthy, M R V; de Bilbao, F; Arsenijevic, D; Giannakopoulos, P; Vallet, P; Richard, D

Paradis, E; Clavel, S; Julien, P; Murthy, M R V; de Bilbao, F; Arsenijevic, D; Giannakopoulos, P; Vallet, P; Richard, D.

Afiliación

Paradis E; Department of Medical Biology, Faculty of Medicine, Laval University, Ste-Foy (PQ), Canada.

Neurobiol Dis ; 15(2): 312-25, 2004 Mar.

Article en En | MEDLINE | ID: mdl-15006701

RESUMEN

Lipoprotein and endothelial lipases are members of the triglyceride lipase gene family. These genes are expressed in the brain, where the encoded proteins are fulfilling functions that have yet to be elucidated. In this study, we examined the distribution of their respective mRNAs in the C57BL/6 mouse brain by in situ hybridization. In control mice, we observed widespread expression of lipoprotein lipase (LPL) mRNA mainly in pyramidal cells of the hippocampus (CA1, CA2 and CA3 areas), in the striatum and in several cortical areas. Endothelial lipase (EL) mRNA expression was restricted to CA3 pyramidal cells of the hippocampus, to ependymal cells in the ventral part of the third ventricle and to some cortical cell layers. To gain insight into the role played by lipases in the brain, neurodegeneration was induced by intraperitoneal injection of kainic acid (KA) or by occlusion of the middle cerebral artery (MCA). Upon injection of KA, a rapid increase in EL mRNA expression was observed in the piriform cortex, hippocampus, thalamus and neocortex. However, the levels of LPL mRNA were unaffected by KA injection. Remarkably, after focal cerebral ischemia, the expression of EL was unaffected whereas a dramatic increase in LPL expression was observed in neocortical areas of the lesioned side of the brain. These results show that LPL and EL transcripts are selectively upregulated in function of the type of brain injury. LPL and EL could thus fulfill a function in the pathophysiological response of the brain to injury.

Asunto(s)

Isquemia Encefálica/enzimología; Encéfalo/enzimología; Regulación Enzimológica de la Expresión Génica/genética; Lipasa/genética; Lipoproteína Lipasa/genética; Degeneración Nerviosa/enzimología; Animales; Encéfalo/fisiopatología; Isquemia Encefálica/fisiopatología; Corteza Cerebral/enzimología; Corteza Cerebral/fisiopatología; Infarto Cerebral/enzimología; Infarto Cerebral/fisiopatología; Cuerpo Estriado/enzimología; Cuerpo Estriado/fisiopatología; Modelos Animales de Enfermedad; Hipocampo/enzimología; Hipocampo/fisiopatología; Infarto de la Arteria Cerebral Media/enzimología; Infarto de la Arteria Cerebral Media/fisiopatología; Ácido Kaínico; Masculino; Ratones; Ratones Endogámicos C57BL; Degeneración Nerviosa/inducido químicamente; Degeneración Nerviosa/fisiopatología; Neurotoxinas/farmacología; Células Piramidales/enzimología; ARN Mensajero/metabolismo; Tiempo de Reacción/fisiología; Regulación hacia Arriba/fisiología

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Regulación Enzimológica de la Expresión Génica / Isquemia Encefálica / Lipasa / Lipoproteína Lipasa / Degeneración Nerviosa Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Neurobiol Dis Asunto de la revista: NEUROLOGIA Año: 2004 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

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