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Possible mechanisms of insulin antinociception.
Anuradha, K; Hota, D; Pandhi, P.
Afiliación
  • Anuradha K; Department of Pharmacology, Post-Graduate Institute of Medical Education & Research, Chandigarh 160012, India. medinst@pgi.chd.nic.in
Article en En | MEDLINE | ID: mdl-14988735
The antinociceptive mechanisms of insulin are not clearly understood. It has been postulated that insulin may act as a neuromodulator. The present study investigates the possible mechanisms of insulin antinociception in mice using the tail flick test. Healthy Swiss male albino mice were treated with insulin and the antinociceptive effect of modulators of 5-HT, NMDA, dopamine, opioids, potassium and calcium channels was tested, followed by blood sugar estimation. All drug doses were given as milligrams per kilogram of body weight 30 min prior to insulin administration, except for para-chlorophenylanine (pCPA), which was given for three consecutive days per orally. Pretreatment with morphine (opioid agonist), 5-Hydroxytryptophan (5-HTP; 5-HT precursor), nicorandil (K(+) channel opener) and nimodipine (Ca(+) channel antagonist) significantly (p < 0.001) potentiated insulin antinociception, whereas naloxone (opioid antagonist), ketanserin (5-HT(2) receptor antagonist), pCPA (5-HT depleter), ondansetron (5-HT(3) receptor antagonist), L-dopa (dopamine precursor), reserpine (dopamine depleter), ketamine (NMDA receptor antagonist) and glibenclamide (K(+) channel blocker) significantly antagonized insulin antinociception (p < 0.001). Results suggest that 5-HT, dopamine, NMDA, opioidergic receptors and potassium and calcium channels play a significant role in insulin analgesia. However, detailed studies on individual mechanisms are necessary.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dimensión del Dolor / Analgésicos / Insulina Límite: Animals Idioma: En Revista: Methods Find Exp Clin Pharmacol Año: 2004 Tipo del documento: Article País de afiliación: India Pais de publicación: España
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dimensión del Dolor / Analgésicos / Insulina Límite: Animals Idioma: En Revista: Methods Find Exp Clin Pharmacol Año: 2004 Tipo del documento: Article País de afiliación: India Pais de publicación: España