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Serum lysophosphatidic acid concentrations measured by dot immunogold filtration assay in patients with acute myocardial infarction.
Chen, X; Yang, X Y; Wang, N D; Ding, C; Yang, Y J; You, Z J; Su, Q; Chen, J H.
Afiliación
  • Chen X; Division of Biochemistry, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China.
Scand J Clin Lab Invest ; 63(7-8): 497-503, 2003.
Article en En | MEDLINE | ID: mdl-14743959
In this study the relation between lysophosphatidic acid (LPA) and myocardial infarction was investigated, the typical and simplified methods for measuring serum LPA concentration by dot immunogold filtration assay (DIFA) based on a polyclonal antibody to LPA were developed, and serum LPA concentrations were measured in 31 patients with acute myocardial infarction (AMI) and 12 controls (blood donors) by DIFA. Serum LPA levels were raised more than twofold 8 h after the onset of AMI. Maximal elevation (10.43 mg/L) was found at 48-72 h following onset and remained higher than the control concentration (1.66 mg/L) 7 days after AMI. The rise in serum LPA concentration in AMI patients suggests that LPA might be involved in AMI-related pathophysiology in the cardiovascular system. The simplified DIFA developed in the present study for measuring serum LPA concentration is convenient and highly sensitive.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lisofosfolípidos / Infarto del Miocardio Límite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Scand J Clin Lab Invest Año: 2003 Tipo del documento: Article Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lisofosfolípidos / Infarto del Miocardio Límite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Scand J Clin Lab Invest Año: 2003 Tipo del documento: Article Pais de publicación: Reino Unido