A repetitive probe for FISH analysis of bovine interphase nuclei.
Genet Sel Evol
; 32(2): 217-25, 2000.
Article
en En
| MEDLINE
| ID: mdl-14736403
The purpose of this study was to generate repetitive DNA sequence probes for the analysis of interphase nuclei by fluorescent in situ hybridisation (FISH). Such probes are useful for the diagnosis of chromosomal abnormalities in bovine preimplanted embryos. Of the seven probes (E1A, E4A, Ba, H1A, W18, W22, W5) that were generated and partially sequenced, five corresponded to previously described Bos taurus repetitive DNA (E1A, E4A, Ba, W18, W5), one probe (W22) shared no homology with other DNA sequences and one (H1A) displayed a significant homology with Rattus norvegicus mRNA for secretin receptor transmembrane domain 3. Fluorescent in situ hybridisation was performed on metaphase bovine fibroblast cells and showed that five of the seven probes hybridised most centromeres (E1A, E4A, Ba, W18, W22), one labelled the arms of all chromosomes (W5) and the H1A probe was specific to three chromosomes (ch14, ch20, and ch25). Moreover, FISH with H1A resulted in interpretable signals on interphase nuclei in 88% of the cases, while the other probes yielded only dispersed overlapping signals.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Genet Sel Evol
Asunto de la revista:
BIOLOGIA
/
GENETICA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Francia