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Plexin signaling hampers integrin-based adhesion, leading to Rho-kinase independent cell rounding, and inhibiting lamellipodia extension and cell motility.
Barberis, D; Artigiani, S; Casazza, A; Corso, S; Giordano, S; Love, C A; Jones, E Y; Comoglio, P M; Tamagnone, Luca.
Afiliación
  • Barberis D; Institute for Cancer Research and Treatment, University of Torino School of Medicine, Candiolo, Italy.
FASEB J ; 18(3): 592-4, 2004 Mar.
Article en En | MEDLINE | ID: mdl-14734633
Plexins encode receptors for semaphorins, molecular signals guiding cell migration, and axon pathfinding. The mechanisms mediating plexin function are poorly understood. Plexin activation in adhering cells rapidly leads to retraction of cellular processes and cell rounding "cell collapse"). Here we show that, unexpectedly, this response does not require the activity of Rho-dependent kinase (ROCK) nor the contraction of F-actin cables. Interestingly, integrin-based focal adhesive structures are disassembled within minutes upon plexin activation; this is followed by actin depolymerization and, eventually, by cellular collapse. We also show that plexin activation hinders cell attachment to adhesive substrates, blocks the extension of lamellipodia, and thereby inhibits cell migration. We conclude that plexin signaling uncouples cell substrate-adhesion from cytoskeletal dynamics required for cell migration and axon extension.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Seudópodos / Citoesqueleto / Transducción de Señal / Antígenos CD / Integrinas / Receptores de Péptidos / Receptores de Superficie Celular / Semaforinas / Proteínas del Tejido Nervioso Límite: Animals Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2004 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Seudópodos / Citoesqueleto / Transducción de Señal / Antígenos CD / Integrinas / Receptores de Péptidos / Receptores de Superficie Celular / Semaforinas / Proteínas del Tejido Nervioso Límite: Animals Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2004 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos