Identification, cloning, and expression of bacteriophage T5 dnk gene encoding a broad specificity deoxyribonucleoside monophosphate kinase (EC 2.7.4.13).
Protein Expr Purif
; 33(2): 166-75, 2004 Feb.
Article
en En
| MEDLINE
| ID: mdl-14711503
The nucleotide sequence corresponding to 13-19.5% of the bacteriophage T5 genome in early region C was determined (GenBank AY 140897). One of the five major single-stranded interruptions (nicks) of bacteriophage T5 DNA was identified at 18.5%. The sequenced region was annotated and the putative functions of some open reading frames were proposed by comparison with databases. The dnk gene, encoding a deoxyribonucleoside monophosphate kinase, was identified using a previously defined N-terminal amino acid sequence. The gene was cloned and expressed in Escherichia coli, the enzyme was purified to homogeneity with high yield using two alternative methods, and the recombinant deoxyribonucleoside monophosphate kinase was found to have the same activity and specificity as the native enzyme.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fagos T
/
Clonación Molecular
/
Fosfotransferasas (Aceptor del Grupo Fosfato)
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Protein Expr Purif
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2004
Tipo del documento:
Article
Pais de publicación:
Estados Unidos