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Somatic activation of oncogenic Kras in hematopoietic cells initiates a rapidly fatal myeloproliferative disorder.
Braun, Benjamin S; Tuveson, David A; Kong, Namie; Le, Doan T; Kogan, Scott C; Rozmus, Jacob; Le Beau, Michelle M; Jacks, Tyler E; Shannon, Kevin M.
Afiliación
  • Braun BS; Department of Pediatrics, University of California, San Francisco, CA 94143, USA.
Proc Natl Acad Sci U S A ; 101(2): 597-602, 2004 Jan 13.
Article en En | MEDLINE | ID: mdl-14699048
RAS mutations are common in myeloid malignancies; however, it is not known whether oncogenic RAS can initiate leukemia. We show that expressing mutant K-Ras(G12D) protein from the endogenous murine locus rapidly induces a fatal myeloproliferative disorder with 100% penetrance characterized by tissue infiltration, hypersensitivity to growth factors, and hyperproliferation. Hematopoietic cells from diseased mice demonstrated increased levels of Ras-GTP, but effector kinases were not constitutively phosphorylated and responded normally to growth factors. Oncogenic RAS is sufficient to initiate myeloid leukemogenesis in mice, and this provides an in vivo system for biologic and preclinical studies.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Genes ras / Trastornos Mieloproliferativos Límite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2004 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Genes ras / Trastornos Mieloproliferativos Límite: Animals Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2004 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos