Immunohistochemical detection of macrophages in the short-finned pilot whale (Globicephala macrorhynchus) and Risso's dolphin (Grampus griseus).
J Comp Pathol
; 130(1): 32-40, 2004 Jan.
Article
en En
| MEDLINE
| ID: mdl-14693122
Macrophages play a central role in the immune system, but few markers are available for their detection in cetaceans. The purpose of the present study, therefore, was to examine the cross-reactivity for two cetacean species (short-finned pilot whale and Risso's dolphin) of four anti-human antibodies (SRA-E5, AM-3K, EBM11 and anti-human lysozyme). The distribution of SRA-E5- and AM-3K-positive cells was similar, both antibodies labelling (1) many resident macrophages in the spleen, lymph nodes, liver, lung, kidney, intestine and dermis, and (2) exudate macrophages in the hepatic interlobular septa. Anti-human lysozyme antibody also labelled both resident and exudate macrophages. However, double immunohistochemistry showed that the majority of AM-3K-positive cells in the spleen and liver were also labelled by SRA-E5; on the other hand, anti-human lysozyme-positive cells did not always correspond with AM-3K-positive cells. Cetacean tissues contained no EBM11-positive cells. The study demonstrated the potential values of SRA-E5, AM-3K and anti-human lysozyme antibody for cetacean macrophage studies.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunohistoquímica
/
Delfines
/
Macrófagos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Comp Pathol
Año:
2004
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido