Interaction of fusogenic peptides with an antisense oligonucleotide in solution and in the presence of micelles: conformational studies.

Laczkó, I; Bottka, S; Tóth, G K; Malvy, C; Bertrand, J-R; Hollósi, M

Laczkó, I; Bottka, S; Tóth, G K; Malvy, C; Bertrand, J-R; Hollósi, M.

Afiliación

Laczkó I; Institute of Biophysics, Biological Research Center, P.O. Box 521, 6701 Szeged, Hungary. laczko@nucleus.szbk.u-szeged.hu

Biochem Biophys Res Commun ; 313(2): 356-61, 2004 Jan 09.

Article en En | MEDLINE | ID: mdl-14684168

RESUMEN

The conformational effect of the interaction between various fusogenic peptides and an 18mer single stranded antisense oligonucleotide (ODN), targeted towards the green fluorescent protein mRNA, has been studied by circular dichroism spectroscopy in water and in the presence of anionic lysolipid micelles. The peptides used were the third helix of Antennapedia homeodomain pAntp-(43-58), the flock house virus FHV-gamma-(364-407) peptide, and its N-terminal gamma1-(364-384) and C-terminal gamma2-(390-407) fragments. The most significant conformational changes were observed in ODN-pAntp-(43-58) and ODN-FHV-gamma2 complexes. The pAntp-(43-58) forms a complex with ODN through electrostatic interaction resulting in profound changes in the conformation of both the peptide and the ODN. In the case of FHV-gamma2 peptide the complex formation takes place without altering the structure of ODN, and the decreased ratio of deltaepsilon208/deltaepsilon222 reflects the insertion of the complexed peptide into the micelle.

Asunto(s)

Oligonucleótidos Antisentido/química; Oligonucleótidos Antisentido/metabolismo; Péptidos/química; Péptidos/metabolismo; Secuencia de Aminoácidos; Aniones; Dicroismo Circular; Proteínas de Homeodominio/química; Proteínas de Homeodominio/genética; Proteínas de Homeodominio/metabolismo; Micelas; Datos de Secuencia Molecular; Oligonucleótidos Antisentido/genética; Fragmentos de Péptidos/química; Fragmentos de Péptidos/genética; Fragmentos de Péptidos/metabolismo; Péptidos/genética; Estructura Secundaria de Proteína; Proteínas Virales/química; Proteínas Virales/genética; Proteínas Virales/metabolismo; Agua/química

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos / Oligonucleótidos Antisentido Idioma: En Revista: Biochem Biophys Res Commun Año: 2004 Tipo del documento: Article País de afiliación: Hungria Pais de publicación: Estados Unidos

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