LexA-binding sequences in Gram-positive and cyanobacteria are closely related.

Mazón, G; Lucena, J M; Campoy, S; Fernández de Henestrosa, A R; Candau, P; Barbé, J

Mazón, G; Lucena, J M; Campoy, S; Fernández de Henestrosa, A R; Candau, P; Barbé, J.

Afiliación

Mazón G; Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.

Mol Genet Genomics ; 271(1): 40-9, 2004 Feb.

Article en En | MEDLINE | ID: mdl-14652736

RESUMEN

The lexA gene of the cyanobacterium Anabaena sp. strain PCC7120 has been cloned by PCR amplification with primers designed after TBLASTN analysis of its genome sequence using the Escherichia coli LexA sequence as a probe. After over-expression in E. coli and subsequent purification, footprinting experiments demonstrated that the Anabaena LexA protein binds to the sequence TAGTACTAATGTTCTA, which is found upstream of its own coding gene. Directed mutagenesis and sequence comparison of promoters of other Anabaena genes, as well as those of several cyanobacteria, allowed us to define the motif RGTACNNNDGTWCB as the LexA box in this bacterial phylum. Substitution of a single nucleotide in this motif present in the Anabena lexA promoter is sufficient to enable it to bind the Bacillus subtilis LexA protein. These data indicate that Cyanobacteria and Gram-positive bacteria are phylogenetically closely related.

Asunto(s)

Proteínas Bacterianas/genética; Proteínas Bacterianas/metabolismo; Cianobacterias/genética; Cianobacterias/metabolismo; Bacterias Grampositivas/genética; Bacterias Grampositivas/metabolismo; Serina Endopeptidasas/genética; Serina Endopeptidasas/metabolismo; Secuencias de Aminoácidos; Secuencia de Aminoácidos; Anabaena/genética; Anabaena/metabolismo; Secuencia de Bases; Sitios de Unión/genética; ADN Bacteriano/genética; ADN Bacteriano/metabolismo; Escherichia coli/genética; Escherichia coli/metabolismo; Proteínas de Escherichia coli/genética; Proteínas de Escherichia coli/metabolismo; Genes Bacterianos; Datos de Secuencia Molecular; Mutagénesis Sitio-Dirigida; Plásmidos/genética; Plásmidos/metabolismo; Regiones Promotoras Genéticas; Unión Proteica; Homología de Secuencia de Aminoácido

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Serina Endopeptidasas / Cianobacterias / Bacterias Grampositivas Idioma: En Revista: Mol Genet Genomics Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2004 Tipo del documento: Article País de afiliación: España Pais de publicación: Alemania

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