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Evaluation of serum insulin-like growth factor binding proteins (IGFBP) in Angus cattle divergently selected for serum IGF-I concentration.
Pagan, M; Davis, M E; Stick, D A; Simmen, R C M; Raney, N E; Tempelman, R J; Ernst, C W.
Afiliación
  • Pagan M; Department of Animal Science, Michigan State University, 1205 Anthony Hall, East Lansing, MI 48824, USA.
Domest Anim Endocrinol ; 25(4): 345-58, 2003 Nov.
Article en En | MEDLINE | ID: mdl-14652135
Postweaning serum insulin-like growth factor-I (IGF-I) concentrations and serum IGF binding proteins (IGFBP) were investigated in 68 (1992 Fall-born) and 84 (1999 Fall-born) Angus cattle selected for either high or low serum IGF-I concentrations since 1989. Relative serum levels of IGFBP were determined by [125I]IGF-I Western ligand blotting. IGFBP species of 38-42, 34, 30, and 24 kDa were identified. The 34 kDa species was identified as IGFBP-2 by immunoblot analysis. No significant line effects were observed for any of the IGFBP. In both 1992 and 1999, heifers had higher IGFBP-2 levels than bulls (P<0.0005). In 1992 calves, relative levels of the 38-42 and 24 kDa species were significantly correlated with serum IGF-I concentration. In 1999 calves, none of the IGFBP were correlated with serum IGF-I, although IGFBP-2 was negatively correlated with several measures of body weight. No significant line effects were observed for growth or serum IGF-I traits in 1992 calves. However, 1999 high line calves had higher serum IGF-I concentrations and body weights than low line calves (P<0.05). In both 1992 and 1999 calves, bulls had higher serum IGF-I concentrations and body weights than heifers (P<0.05). Thus, while selection for high versus low serum IGF-I concentrations has resulted in divergence between the selection lines and also in changes in body weights, it has not resulted in changes in serum IGFBP levels. Furthermore, circulating IGFBP-2 appears to be higher in heifers than in bulls, and also appears to be negatively correlated with body weights.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Selección Genética / Factor I del Crecimiento Similar a la Insulina / Bovinos / Proteínas de Unión a Factor de Crecimiento Similar a la Insulina Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Domest Anim Endocrinol Asunto de la revista: ENDOCRINOLOGIA / MEDICINA VETERINARIA Año: 2003 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Selección Genética / Factor I del Crecimiento Similar a la Insulina / Bovinos / Proteínas de Unión a Factor de Crecimiento Similar a la Insulina Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Domest Anim Endocrinol Asunto de la revista: ENDOCRINOLOGIA / MEDICINA VETERINARIA Año: 2003 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos