Measurement of glutathione in activated sludges.
Water Res
; 38(1): 236-44, 2004 Jan.
Article
en En
| MEDLINE
| ID: mdl-14630122
Thermal, electric, mechanical or oxidative stress seem a promising way to reduce the production of excess activated sludge during biological wastewater treatment. However, the adaptation and the resistance of the sludge microbial ecosystem to stress conditions is a major question as it may definitively limit the effect of some treatments. Defence mechanisms developed by aerobic organisms, in particular, in response to oxidative stress involve various antioxidant activities and compounds such as glutathione. An HPLC method was developed for measuring reduced and total glutathione (GSH and GSHt) in perchloric acid sludge extracts. The method was sensitive, highly specific and validated for linearity, precision and recovery. Considering the extraction yield and the oxidation of GSH during extract storage, the measured GSH concentration was estimated to represent 60% of the GSH content from activated sludges. GSHt ranged from 0.32 to 3.34micromolg(-1) volatile solids and the GSH/GSHt ratio ranged from 32% to 91%. Measurements performed on sludges stressed in precise conditions selected to reach a reduction of sludge production showed a decrease of GSH and GSHt concentrations with thermal, mechanical, electric and ozone stress.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aguas del Alcantarillado
/
Eliminación de Residuos Líquidos
/
Estrés Oxidativo
/
Glutatión
Idioma:
En
Revista:
Water Res
Año:
2004
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido