Direct interaction between alpha-actinin and hepatitis C virus NS5B.
FEBS Lett
; 554(3): 289-94, 2003 Nov 20.
Article
en En
| MEDLINE
| ID: mdl-14623081
It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two-hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, alpha-actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic analysis. Experiments with deletion mutants showed that domains NS5B(84-95), NS5B(466-478), and alpha-actinin(621-733) are responsible for the interaction. Studies of the HCV subgenomic replicon system with small interference RNA indicate that alpha-actinin is essential for HCV RNA replication. Our results suggest alpha-actinin may be a component of the HCV replication complex.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Actinina
/
ARN Polimerasas Dirigidas por ADN
/
Hepacivirus
Límite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2003
Tipo del documento:
Article
Pais de publicación:
Reino Unido