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Direct interaction between alpha-actinin and hepatitis C virus NS5B.
Lan, Shuiyun; Wang, Hua; Jiang, Hong; Mao, Hongxia; Liu, Xiaoying; Zhang, Xiaonan; Hu, Yunwen; Xiang, Li; Yuan, Zhenghong.
Afiliación
  • Lan S; Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.
FEBS Lett ; 554(3): 289-94, 2003 Nov 20.
Article en En | MEDLINE | ID: mdl-14623081
It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two-hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, alpha-actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic analysis. Experiments with deletion mutants showed that domains NS5B(84-95), NS5B(466-478), and alpha-actinin(621-733) are responsible for the interaction. Studies of the HCV subgenomic replicon system with small interference RNA indicate that alpha-actinin is essential for HCV RNA replication. Our results suggest alpha-actinin may be a component of the HCV replication complex.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Actinina / ARN Polimerasas Dirigidas por ADN / Hepacivirus Límite: Animals / Humans Idioma: En Revista: FEBS Lett Año: 2003 Tipo del documento: Article Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Actinina / ARN Polimerasas Dirigidas por ADN / Hepacivirus Límite: Animals / Humans Idioma: En Revista: FEBS Lett Año: 2003 Tipo del documento: Article Pais de publicación: Reino Unido