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Analyses of carcinogenic aromatic amines released from harmful azo colorants by Streptomyces SP. SS07.
Bhaskar, Malayappan; Gnanamani, Arumugam; Ganeshjeevan, Rama Jeevan; Chandrasekar, Raghavan; Sadulla, Sayeed; Radhakrishnan, Ganga.
Afiliación
  • Bhaskar M; Eco Analytical Laboratory, Central Leather Research Institute, Adyar, Chennai 600020, Tamil Nadu, India.
J Chromatogr A ; 1018(1): 117-23, 2003 Nov 07.
Article en En | MEDLINE | ID: mdl-14582632
Extracellular fluid protein (ECFP) of Streptomyces species SS07 has been used to reduce water soluble azo dyes and the carcinogenic amines released have been compared with that from chemical reduction. The effect of temperature, pH and contact time on the recovery of amines using ECFP was studied. The ECFP releases carcinogenic amines at a pH of 9.2 and a temperature of 37 degrees C for a contact period of 24 h. The reduction products were analyzed with HPLC and their structures confirmed by LC-MS and GC-MS. It was observed that both the ECFP and chemical reduction methods released similar type of amine products. In the case of dye samples, compared to chemical reduction, 5-20% increase in the release of carcinogenic amines by ECFP was observed. The percentage of amine products released by chemical reduction was higher for leather garment samples compared to ECFP treatment.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Compuestos Azo / Carcinógenos / Aminas Idioma: En Revista: J Chromatogr A Año: 2003 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Compuestos Azo / Carcinógenos / Aminas Idioma: En Revista: J Chromatogr A Año: 2003 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos