[A novel laboratory method of isolation and purification of tobramycin]. / Razrabotka novogo laboratornogo metoda vydeleniia i ochistki tobramitsina.
Antibiot Khimioter
; 48(6): 3-6, 2003.
Article
en Ru
| MEDLINE
| ID: mdl-14558411
A new laboratory method for isolation and purification of tobramycin by using extraction of a tobramycin derivative with benzaldehyde by methylene chloride, subsequent hydrolysis of azomethine and recrystallization of the formed tobramycin sulfate from solution of sulfuric acid in methanol was developed. The method allows to exclude the stage of chromatographic purification of tobramycin, to reduce the time of the process realization from 120-125 h to 15-20 h, to increase the yield of the target product from 37-40% to 60-65% without decreasing the product quality, to exclude a number of large-size and expensive equipment and to ensure high reproducibility of the technology.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tobramicina
/
Antibacterianos
Idioma:
Ru
Revista:
Antibiot Khimioter
Asunto de la revista:
TERAPIA POR MEDICAMENTOS
Año:
2003
Tipo del documento:
Article
Pais de publicación:
Rusia