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[A novel laboratory method of isolation and purification of tobramycin]. / Razrabotka novogo laboratornogo metoda vydeleniia i ochistki tobramitsina.
Protasov, V L; Vydrin, A F; Mokrushina, G A; Kodess, M I; Mikhailov, V A; Sadovoi, N V.
Afiliación
  • Protasov VL; Center of Military Technical Problems of Biological Protection, Research Institute of Microbiology, Ministry of Defense of the Russian Federation, Ural State Technical University, Institute of Organic Synthesis, Ural Branch, Russian Academy of Sciences.
Antibiot Khimioter ; 48(6): 3-6, 2003.
Article en Ru | MEDLINE | ID: mdl-14558411
A new laboratory method for isolation and purification of tobramycin by using extraction of a tobramycin derivative with benzaldehyde by methylene chloride, subsequent hydrolysis of azomethine and recrystallization of the formed tobramycin sulfate from solution of sulfuric acid in methanol was developed. The method allows to exclude the stage of chromatographic purification of tobramycin, to reduce the time of the process realization from 120-125 h to 15-20 h, to increase the yield of the target product from 37-40% to 60-65% without decreasing the product quality, to exclude a number of large-size and expensive equipment and to ensure high reproducibility of the technology.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tobramicina / Antibacterianos Idioma: Ru Revista: Antibiot Khimioter Asunto de la revista: TERAPIA POR MEDICAMENTOS Año: 2003 Tipo del documento: Article Pais de publicación: Rusia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tobramicina / Antibacterianos Idioma: Ru Revista: Antibiot Khimioter Asunto de la revista: TERAPIA POR MEDICAMENTOS Año: 2003 Tipo del documento: Article Pais de publicación: Rusia