Human chymase stimulates Ca2+ signaling in human polymorphonuclear cells.
Immunol Lett
; 89(2-3): 161-5, 2003 Oct 31.
Article
en En
| MEDLINE
| ID: mdl-14556974
Human chymase is known to function as a chemoattractant for human leukocytes. To investigate the mechanism of the chymase-induced cell migration, change in intracellular calcium concentration ([Ca(2+)]i) was examined in human polymorphonuclear (PMN) cells using Fluo-3 as a fluorescent Ca(2+) indicator. Treatment of PMN cells with human chymase caused [Ca(2+)]i elevation in a concentration-dependent manner. Depletion of extracellular Ca(2+) from the medium partially attenuated the chymase-induced [Ca(2+)]i increase, showing that both Ca(2+) influx and Ca(2+) release from internal stores might be involved in the [Ca(2+)]i response. Pretreatment of the cells with pertussis toxin completely blocked the chymase-induced [Ca(2+)]i signal, suggesting an involvement of G protein in the chymase-mediated [Ca(2+)]i elevation. The data in the present study raise the possibility that the chymase-induced cell migration is mediated by the [Ca(2+)]i elevation, which might be caused by stimulation of a G-protein-coupled receptor such as protease-activated receptors (PARs).
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Serina Endopeptidasas
/
Calcio
/
Señalización del Calcio
/
Neutrófilos
Límite:
Humans
Idioma:
En
Revista:
Immunol Lett
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Países Bajos