Photoregulation of deacylation rate of acyl trypsin derived from photoresponsive inverse substrate.

Sekizaki, Haruo; Kumagai, Asako; Itoh, Kunihiko; Toyota, Eiko; Horita, Kiyoshi; Noguchi, Yukari; Tanizawa, Kazutaka

Sekizaki, Haruo; Kumagai, Asako; Itoh, Kunihiko; Toyota, Eiko; Horita, Kiyoshi; Noguchi, Yukari; Tanizawa, Kazutaka.

Afiliación

Sekizaki H; Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan. sekizaki@hoku-iryo-u.ac.jp

Bioorg Med Chem Lett ; 13(21): 3809-12, 2003 Nov 03.

Article en En | MEDLINE | ID: mdl-14552785

RESUMEN

The acyl trypsin was prepared by use of an inverse substrate, which is comprise of a photoresponsive 4-phenylazobenzoyl moiety. The acyl group in acyl trypsin has been shown to isomerize from trans-form (4t-trypsin) to cis-form (4c-trypsin)/from cis-form to trans-form by irradiation of UV-vis light. The deacylation rate of the cis-form (4c-trypsin) has been shown to be 18.6 times faster than that of the trans-form (4t-trypsin).

Asunto(s)

Tripsina/química; Remoción de Radical Alquila; Hidrólisis; Cinética; Fotoquímica; Relación Estructura-Actividad; Rayos Ultravioleta

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tripsina Idioma: En Revista: Bioorg Med Chem Lett Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2003 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

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