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Detection and evaluation of NO stores in awake rats.
Mashina, S Yu; Vanin, A F; Serezhenkov, V A; Kubrina, L N; Malenkova, I V; Malyshev, I Yu; Manukhina, E B.
Afiliación
  • Mashina SY; Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow.
Bull Exp Biol Med ; 136(1): 26-9, 2003 Jul.
Article en En | MEDLINE | ID: mdl-14534603
We demonstrated and substantiated the possibility of detection and evaluation of NO stores in freely moving awake rats. NO stores were created by administering NO donor or by heat shock and were then detected by hypotensive reaction to diethyldithiocarbamate (blood pressure monitoring) under conditions of NO synthase inhibition. Electron paramagnetic resonance revealed NO release from its stores by incorporation into paramagnetic mononitrosyl-iron complexes with diethyldithiocarbamate. Five hours after administration of NO donor or heat shock diethyldithiocarbamate induced a blood pressure drop and the appearance of electron paramagnetic resonance signals from the mononitrosyl-iron-diethyldithiocarbamate complex in rat heart, liver, kidneys, and brain. The hypotensive reaction to diethyldithiocarbamate and electron paramagnetic resonance signals were absent in control rats.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vigilia / Óxido Nítrico Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Bull Exp Biol Med Año: 2003 Tipo del documento: Article Pais de publicación: Estados Unidos
Buscar en Google
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Imprimir
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vigilia / Óxido Nítrico Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Bull Exp Biol Med Año: 2003 Tipo del documento: Article Pais de publicación: Estados Unidos