Characterization of polysaccharide production of haemophilus influenzae Type b and its relationship to bacterial cell growth.
Appl Biochem Biotechnol
; 110(2): 91-100, 2003 Aug.
Article
en En
| MEDLINE
| ID: mdl-14515024
Haemophilus influenzae type b (Hib) causes invasive infections in infants and young children. Vaccines consisting of Hib capsular polysaccharide (polymer of ribosylribitol phosphate [PRP]) conjugated to a protein are effective in the prevention of such infections. The production of capsular polysaccharide type b was studied in three cultivation conditions: single, glucose pulse, and repeated batch. Specific polysaccharide production (Yp/x) was calculated for all experiments, showing the following values: 67 (single-batch cultivation), 71 (glucose pulse), 75 (repeated-batch cultivation, first batch), and 87 mg of PRP/g of dry cell weight (DCW) (repeated-batch cultivation, second batch). Biomass concentration reached approximately 1.8 g of DCW/L, while polysaccharide concentration was about approximately 132 mg/L in the three fermentation runs. Polysaccharide synthesis is associated with cell growth in all studied conditions as established by Kono's analysis and Luedeking-Piret's model.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos Bacterianos
/
Vacunas contra Haemophilus
/
Haemophilus influenzae tipo b
/
Infecciones por Haemophilus
Idioma:
En
Revista:
Appl Biochem Biotechnol
Año:
2003
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos