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Induction and intracellular localization of HSP-72 after renal ischemia.
Van Why, S K; Hildebrandt, F; Ardito, T; Mann, A S; Siegel, N J; Kashgarian, M.
Afiliación
  • Van Why SK; Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06510.
Am J Physiol ; 263(5 Pt 2): F769-75, 1992 Nov.
Article en En | MEDLINE | ID: mdl-1443167
To determine whether heat shock proteins (HSPs) might be active in cellular recovery following transient ischemia, we examined rat kidneys for 70-kDa HSP (HSP-70) mRNA expression, protein elaboration, and intracellular localization after 45 min of renal ischemia and reflow of 15 min, 2, 6, and 24 h. Inducible HSP-70 mRNA is present at 15 min of reperfusion, peaks between 2 and 6 h, and falls by 24 h. Inducible 72-kDa HSP (HSP-72) protein accumulates progressively through 24 h and is found in both soluble and microsomal fractions following ischemia. Within proximal tubules, immunofluorescent localization of HSP-72 is restricted to the apical domain at 15 min, is dispersed through the cytoplasm in a vesicular pattern at 2 and 6 h, and has migrated away from the apical domain at 24 h. A portion of the vesicular HSP-72 is associated with lysosomes; no intranuclear HSP-72 is detected. The course of mRNA induction, protein elaboration, and HSP-72 localization coincides with previously described changes in proximal tubule morphology and polarity following sublethal ischemic injury. HSP-72 may be instrumental in cellular remodeling and restitution of epithelial polarity during recovery from ischemic renal injury.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Circulación Renal / Proteínas de Choque Térmico / Membranas Intracelulares / Isquemia Límite: Animals Idioma: En Revista: Am J Physiol Año: 1992 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Circulación Renal / Proteínas de Choque Térmico / Membranas Intracelulares / Isquemia Límite: Animals Idioma: En Revista: Am J Physiol Año: 1992 Tipo del documento: Article Pais de publicación: Estados Unidos