Labelling of liposomes with intercalating perylene fluorescent dyes.
Biochim Biophys Acta
; 1110(2): 151-7, 1992 Oct 05.
Article
en En
| MEDLINE
| ID: mdl-1390843
The high fluorescent potential and the exceptional photostability of lipophilic derivatives of perylene-3,4:9,10-bis(dicarboximides) are utilized for the fluorescence-labelling of liposomes. The preparation of the liposomes is effected by supersonic starting from a lipid mixture consisting of the matrix lipids soy lecithin, cholesterol, alpha-tocopherol and the perylene dyes. From a multitude of perylene derivatives investigated only those are optimally incorporated into the bilayer membrane of unilamellar liposomes which are substituted at both nitrogen atoms by one or two linear hydrocarbon groups. In order to attain an optimal fluorescent quantum yield, about 200 to 300 dye molecules can be incorporated per liposome. The liposomes thus obtained have a diameter of about 70 to 80 nm, are homogeneous and may be stored for more than seven months. Neither the fluorescent properties nor the stability of these liposomes are influenced by the additional incorporation of various ara C-derivatives and lipophilic anchor groups which subsequently enable the coupling of antibodies to the liposomes. As the water-insoluble perylene dyes are incorporated into the bilayer membrane, the aqueous inner volume of the liposomes remains available for a further utilization.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Perileno
/
Colorantes Fluorescentes
/
Liposomas
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1992
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Países Bajos