Two techniques for electron opaque staining of elastic fibres using tannic acid in fresh and formalin fixed tissue.
J Clin Pathol
; 45(7): 633-5, 1992 Jul.
Article
en En
| MEDLINE
| ID: mdl-1381384
Two electron microscopic staining techniques, one using tannic acid-glutaraldehyde as a fixative, and the other using tannic acid-uranyl acetate solution as a stain on ultra-thin sections of glutaraldehyde fixed material, were directly compared for elastic fibre staining on several human and animal tissues. Various concentrations of tannic acid were compared using both techniques. The two techniques were also compared on formalin fixed tissues. The use of tannic acid-uranyl acetate solution as a stain on processed tissue is by far the more consistent technique and achieves equally good results on glutaraldehyde or formalin fixed tissue. It is suggested that the use of the term tannic acid technique/method should be reserved for this particular method to achieve a meaningful interpretation of results in scientific papers.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Coloración y Etiquetado
/
Fijación del Tejido
/
Taninos Hidrolizables
/
Tejido Elástico
/
Formaldehído
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Clin Pathol
Año:
1992
Tipo del documento:
Article
Pais de publicación:
Reino Unido