Rapid and simple method for the identification of apolipoprotein E isomorphic phenotypes from whole serum.

Kohlmeier, M; Drossel, H J; Sinha, P; Köttgen, E

Kohlmeier, M; Drossel, H J; Sinha, P; Köttgen, E.

Afiliación

Kohlmeier M; Universitätsklinikum Rudolf Virchow, Freien Universität, Institut für Klinische Chemie und Biochemie, Berline, Germany.

Electrophoresis ; 13(4): 258-61, 1992 Apr.

Article en En | MEDLINE | ID: mdl-1378389

RESUMEN

For the identification of apolipoprotein E isomorphic phenotypes, fresh or thawed serum was analyzed without prior delipidation or other pretreatment. Using 5% polyacrylamide gels with a 40 mm interelectrode distance, the isoforms were separated by isoelectric focusing in immobilized pH gradients ranging from pH 5 to 6.5, and transferred onto polyvinylidene difluoride membranes by contact blotting for 1 h. The apolipoprotein E isoforms were identified following immunostaining. The electrophoresis required less than 2 h and the entire procedure could be completed within 6 h.

Asunto(s)

Apolipoproteínas E/genética; Anticuerpos Monoclonales/aislamiento & purificación; Apolipoproteínas E/sangre; Humanos; Concentración de Iones de Hidrógeno; Immunoblotting; Focalización Isoeléctrica; Fenotipo; Coloración y Etiquetado; Factores de Tiempo

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apolipoproteínas E Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Electrophoresis Año: 1992 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links