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In vivo degradation of fetal wound hyaluronic acid results in increased fibroplasia, collagen deposition, and neovascularization.
Mast, B A; Haynes, J H; Krummel, T M; Diegelmann, R F; Cohen, I K.
Afiliación
  • Mast BA; Division of Plastic and Reconstructive Surgery, Medical College of Virginia, Richmond.
Plast Reconstr Surg ; 89(3): 503-9, 1992 Mar.
Article en En | MEDLINE | ID: mdl-1371361
Fetal tissue repair occurs without acute inflammation, prominent fibroplasia, or marked neovascularization. The fetal wound extracellular matrix is rich in hyaluronic acid (HA), while collagen is deposited in an organized normal dermal pattern. In various biologic systems, including regeneration and development, the controlled accumulation and subsequent degradation of hyaluronic acid is associated with distinct cellular and matrix events. Therefore, it is hypothesized that the abundance of hyaluronic acid in fetal wounds may influence cellular and/or matrix events such that tissue repair is highly organized and adult-like scarring does not occur. To test this hypothesis, the hyaluronic acid content of fetal rabbit wounds was reduced by specific degradation with Streptomyces hyaluronidase. Control wounds were treated with either enzyme buffer (n = 12) or denatured enzyme solution (n = 8) and exhibited a normal fetal healing response with scattered peripheral fibroblasts, a matrix of hyaluronic acid, and no infiltrating collagen. In marked contrast, the hyaluronidase-treated wounds (n = 14) demonstrated increased fibroblast infiltration, collagen deposition, and capillary formation. A significant reduction in the hyaluronic acid content of the hyaluronidase-treated wounds was confirmed biochemically. Since the degradation of hyaluronic acid resulted in an altered healing response, this study demonstrates that hyaluronic acid affects the cellular and matrix events in fetal healing and may be partially responsible for the unique qualities of this regenerative repair process.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Colágeno / Feto / Ácido Hialurónico / Neovascularización Patológica Límite: Animals Idioma: En Revista: Plast Reconstr Surg Año: 1992 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Colágeno / Feto / Ácido Hialurónico / Neovascularización Patológica Límite: Animals Idioma: En Revista: Plast Reconstr Surg Año: 1992 Tipo del documento: Article Pais de publicación: Estados Unidos