Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers.

Mignot, G; Faure, T; Ganne, V; Arbeille, B; Pavirani, A; Romet-Lemonne, J L

Mignot, G; Faure, T; Ganne, V; Arbeille, B; Pavirani, A; Romet-Lemonne, J L.

Afiliación

Mignot G; Centre National de Transfusion Sanguine, Les Ulis, France.

Cytotechnology ; 4(2): 163-71, 1990 Sep.

Article en En | MEDLINE | ID: mdl-1370026

RESUMEN

Recombinant Chinese hamster ovary cells producing Von Willebrand factor have been successfully grown in gelatin macroporous microcarriers (Cultispher-G). Serum-free cultures were maintained in 1, 4, and 10 liter fermentors for more than two months. Comparative studies with Cytodex-3 microcarriers have been performed in 1 liter fermentors. The lower specific Von Willebrand factor productivity of CHO cells cultivated on Cultispher-G were offset by higher cell densities (10(7) - 2 x 10(7) cells/ml). Volumetric Von Willebrand factor productivity was influenced by oxygen concentration, and remained stable during scale-up from 1 to 10 liter fermentors.

Asunto(s)

Microesferas; Factor de von Willebrand/biosíntesis; Animales; Biotecnología/instrumentación; Biotecnología/métodos; Línea Celular; Dextranos; Gelatina; Microscopía Electrónica de Rastreo; Permeabilidad; Proteínas Recombinantes/biosíntesis

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor de von Willebrand / Microesferas Límite: Animals Idioma: En Revista: Cytotechnology Asunto de la revista: BIOTECNOLOGIA / GENETICA Año: 1990 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos

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