Continuous production of large amounts of monoclonal immunoglobulins in hollow fibers using protein-free medium.
Cytotechnology
; 10(1): 33-41, 1992.
Article
en En
| MEDLINE
| ID: mdl-1369103
The performance of a protein-free medium was compared in culture flasks with a serum-supplemented medium and with a serum free medium in terms of cell growth and monoclonal antibody production by a murine hybridoma. We present results of continuous production in hollow fiber culture systems using serum-free medium and protein-free medium. In protein-free medium, it has been possible to produce large quantities of monoclonal antibody with a productivity similar to that obtained in serum-free medium. After a two steps purification process, monoclonal antibodies were characterized by SDS-PAGE, High Performance Size Exclusion Chromatography and Free Solution Capillary Electrophoresis. SDS-PAGE and high performance chromatography analysis have showed that purified monoclonal antibodies produced in serum-free medium or protein-free medium were similar. Furthermore, Capillary Electrophoresis characterization revealed that both MAbs were constituted by three isoforms with equivalent electrophoretic mobilities.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunoglobulina G
/
Anticuerpos Monoclonales
Límite:
Animals
Idioma:
En
Revista:
Cytotechnology
Asunto de la revista:
BIOTECNOLOGIA
/
GENETICA
Año:
1992
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos