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High resolution two-dimensional protein gel evidence of differential gene expression during Entamoeba encystation.
Bond, V C; Beanan, M J; Bailey, G B.
Afiliación
  • Bond VC; Department of Biochemistry, Morehouse School of Medicine, Atlanta, GA 30010.
Arch Med Res ; 23(2): 11-3, 1992.
Article en En | MEDLINE | ID: mdl-1340270
As an initial step of investigation into the regulatory mechanisms of encystation in Entamoeba, we compared the protein profiles of newly formed cysts and trophozoites of E. invadens using high resolution two-dimensional PAGE and digitized video image analysis of silver stained gels. A total of 155 proteins unique to trophozoites and a total of 72 proteins unique to cysts were detected. Five of the most prominent cyst specific proteins were identified as candidates for further study. These results imply that extensive changes in gene expression accompany the progression of this parasite through its life cycle.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroforesis en Gel Bidimensional / Proteínas Protozoarias / Regulación de la Expresión Génica / Entamoeba Límite: Animals Idioma: En Revista: Arch Med Res Asunto de la revista: MEDICINA Año: 1992 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Electroforesis en Gel Bidimensional / Proteínas Protozoarias / Regulación de la Expresión Génica / Entamoeba Límite: Animals Idioma: En Revista: Arch Med Res Asunto de la revista: MEDICINA Año: 1992 Tipo del documento: Article Pais de publicación: Estados Unidos