Inhibitory effect of insulin and cytoplasmic factor(s) on brain (Na(+) + K+) ATPase.

Catalán, R E; Martínez, A M; Aragonés, M D; Fernández, I; Miguel, B G

Catalán, R E; Martínez, A M; Aragonés, M D; Fernández, I; Miguel, B G.

Afiliación

Catalán RE; Departamento de Biología Molecular, Universidad Autónoma de Madrid, Spain.

Neurosci Res ; 13(2): 139-45, 1992 Mar.

Article en En | MEDLINE | ID: mdl-1316592

RESUMEN

(Na+ + K+)ATPase activity in cerebral cortex was modulated by insulin action depending on the Mg2+ concentration. Thus, in homogenates in the presence of 1-3 mM Mg2+, insulin stimulated the enzyme, whereas in the presence of 4-6 mM Mg2+ inhibition was observed. Exposure of synaptosomal membranes to the soluble fraction resulted in inhibition of ATPase activity in a dose-dependent manner. The inhibitory effect of insulin was regulated by a cytoplasmic factor in a dose-dependent manner. Similar variations to those obtained with a crude synaptosomal fraction were obtained by using a partially purified ATPase. These results indicated the importance of soluble factors in the modulation of ATPase by insulin and add more evidence in support for a role of insulin as a neuromodulator.

Asunto(s)

Corteza Cerebral/enzimología; Citoplasma/fisiología; Insulina/farmacología; Magnesio/farmacología; ATPasa Intercambiadora de Sodio-Potasio/antagonistas & inhibidores; Sinaptosomas/enzimología; Animales; Relación Dosis-Respuesta a Droga; Cinética; Masculino; Ratones; Sinaptosomas/efectos de los fármacos

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sinaptosomas / Corteza Cerebral / ATPasa Intercambiadora de Sodio-Potasio / Citoplasma / Insulina / Magnesio Límite: Animals Idioma: En Revista: Neurosci Res Asunto de la revista: NEUROLOGIA Año: 1992 Tipo del documento: Article País de afiliación: España Pais de publicación: Irlanda

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