Female-specific DNA sequences in geese.
Br Poult Sci
; 44(3): 359-64, 2003 Jul.
Article
en En
| MEDLINE
| ID: mdl-12964618
1. The OPAE random primers (Operon Technologies, Inc., CA) were used for random amplified polymorphic DNA (RAPD) fingerprinting in Chinese, White Roman and Landaise geese. One of these primers, OPAE-06, produced a 938-bp sex-specific fragment in all females and in no males of Chinese geese only. 2. A novel female-specific DNA sequence in Chinese goose was cloned and sequenced. Two primers, CGSex-F and CGSex-R, were designed in order to amplify a 912-bp sex-specific polymerase chain reaction (PCR) fragment on genomic DNA from female geese. 3. It was shown that a simple and effective PCR-based sexing technique could be used in the three goose breeds studied. 4. Nucleotide sequencing of the sex-specific fragments in White Roman and Landaise geese was performed and sequence differences were observed among these three breeds.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Análisis para Determinación del Sexo
/
ADN
/
Técnica del ADN Polimorfo Amplificado Aleatorio
/
Gansos
Límite:
Animals
Idioma:
En
Revista:
Br Poult Sci
Año:
2003
Tipo del documento:
Article
País de afiliación:
Taiwán
Pais de publicación:
Reino Unido