A chromatographic approach to analyze dansyl amino acid-HP-beta-CD association using macrocyclic antibiotic as the stationary phase.
J Chromatogr Sci
; 41(6): 311-6, 2003 Jul.
Article
en En
| MEDLINE
| ID: mdl-12935303
The retention mechanism for a series of D,L-dansyl amino acids in high-performance liquid chromatography is investigated using a teicoplanin stationary phase and hydroxypropyl-beta-cyclodextrin (HP-beta-CD) as the mobile phase additive (0-16mM). A theoretical treatment is developed to determine the HP-beta-CD influence on the equilibrium between the teicoplanin phase and the aqueous medium, respectively. From the experimental data, the association constants of the D,L-dansyl amino acids-HP-beta-CD inclusion complexes are determined and discussed in relation to the enantiomer structure. A thermodynamic study confirms that both the retention and complexation mechanisms are independent of the dansyl amino acid molecular structure and its absolute carbon configuration.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos de Dansilo
/
Aminoácidos
/
Antibacterianos
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
J Chromatogr Sci
Año:
2003
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos