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Cell biology of laryngeal epithelial defenses in health and disease: further studies.
Johnston, Nikki; Bulmer, David; Gill, Gulnaz A; Panetti, Marguerite; Ross, Peter E; Pearson, Jeffrey P; Pignatelli, Massimo; Axford, Sophie E; Dettmar, Peter W; Koufman, James A.
Afiliación
  • Johnston N; Center for Voice Disorders of Wake Forest University, Department of Otolaryngology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157-1034, USA.
Ann Otol Rhinol Laryngol ; 112(6): 481-91, 2003 Jun.
Article en En | MEDLINE | ID: mdl-12834114
This is the second annual report of an international collaborative research group that is examining the cellular impact of laryngopharyngeal reflux (LPR) on laryngeal epithelium. The results of clinical and experimental studies are presented. Carbonic anhydrase (CA), E-cadherin, and MUC gene expression were analyzed in patients with LPR, in controls, and in an in vitro model. In patients with LPR, we found decreased levels of CAIII in vocal fold epithelium and increased levels in posterior commissure epithelium. The experimental studies confirm that laryngeal CAIII is depleted in response to reflux. Also, cell damage does occur well above pH 4.0. In addition, E-cadherin (transmembrane cell surface molecules, which have a key function in epithelial cell adhesion) was not present in 37% of the LPR laryngeal specimens. In conclusion, the laryngeal epithelium lacks defenses comparable to those in esophageal epithelium, and these differences may contribute to the increased susceptibility of laryngeal epithelium to reflux-related injury.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reflujo Gastroesofágico / Cadherinas / Enfermedades de la Laringe / Anhidrasas Carbónicas / Mucosa Laríngea / Mucinas Tipo de estudio: Etiology_studies Límite: Animals / Humans Idioma: En Revista: Ann Otol Rhinol Laryngol Año: 2003 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reflujo Gastroesofágico / Cadherinas / Enfermedades de la Laringe / Anhidrasas Carbónicas / Mucosa Laríngea / Mucinas Tipo de estudio: Etiology_studies Límite: Animals / Humans Idioma: En Revista: Ann Otol Rhinol Laryngol Año: 2003 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos