The role of CD36 in peripheral nerve remyelination after crush injury.

Eto, Masaki; Yoshikawa, Hiroo; Fujimura, Harutoshi; Naba, Ichiro; Sumi-Akamaru, Hisae; Takayasu, Satoshi; Itabe, Hiroyuki; Sakoda, Saburo

Eto, Masaki; Yoshikawa, Hiroo; Fujimura, Harutoshi; Naba, Ichiro; Sumi-Akamaru, Hisae; Takayasu, Satoshi; Itabe, Hiroyuki; Sakoda, Saburo.

Afiliación

Eto M; Department of Neurology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka Suita, Osaka 565-0871, Japan.

Eur J Neurosci ; 17(12): 2659-66, 2003 Jun.

Article en En | MEDLINE | ID: mdl-12823473

RESUMEN

We previously demonstrated that the deficiency of class A macrophage scavenger receptor type I/II was involved in the delayed phagocytosis of degraded myelin by macrophages in class A macrophage scavenger receptor type I/II knockout mice after crush injury of the sciatic nerve [Naba et al. (2000) Exp. Neurol., 166, 83-89]. In order to elucidate the role of CD36, one of the scavenger receptors, here we inflicted crush injury to the sciatic nerves of CD36 knockout mice and investigated the remyelination after crush injury in comparison with that of class A macrophage scavenger receptor type I/II knockout mice. Although we previously reported a lot of onion-bulbs in class A macrophage scavenger receptor type I/II knockout mice at 3 weeks, the number of onion-bulbs was limited both in CD36 knockout mice and wild-type mice. In the morphometry, the remyelination was seriously delayed, and the infiltrating macrophages into the nerve fascicles were quite frequent in CD36 knockout mice compared with wild-type mice at 3 and 6 weeks postinjury. The immunohistochemistry with the monoclonal antibody reacted with oxidized phosphatidylcholine and oil red O staining were positive in wild-type mice, but were negative in CD36 knockout mice, suggesting that the oxidation of phosphatidylcholine and the generation of neutral lipids in macrophages were disturbed in CD36 knockout mice. We hypothesize that the delayed phagocytosis by macrophages and the defect in reuse of lipids from degraded myelin are related to seriously delayed remyelination and a small number of onion-bulbs in CD36 knockout mice.

Asunto(s)

Antígenos CD36/metabolismo; Fibras Nerviosas Mielínicas/metabolismo; Neuropéptidos; Enfermedades del Sistema Nervioso Periférico/metabolismo; Nervio Ciático/metabolismo; Nervio Ciático/fisiopatología; Animales; Antígenos CD36/genética; Recuento de Células; Modelos Animales de Enfermedad; Femenino; Guanilato-Quinasas; Inmunohistoquímica/métodos; Lectinas/metabolismo; Macrófagos/metabolismo; Macrófagos/patología; Proteínas de la Membrana; Ratones; Ratones Endogámicos C57BL; Ratones Noqueados; Vaina de Mielina/metabolismo; Vaina de Mielina/patología; Compresión Nerviosa/métodos; Fibras Nerviosas Mielínicas/patología; Proteínas Nucleares/metabolismo; Enfermedades del Sistema Nervioso Periférico/patología; Receptores Depuradores de Clase A; Nervio Ciático/lesiones; Nervio Ciático/patología; Coloración y Etiquetado/métodos; Factores de Tiempo; Factores de Transcripción/metabolismo

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervio Ciático / Neuropéptidos / Enfermedades del Sistema Nervioso Periférico / Antígenos CD36 / Fibras Nerviosas Mielínicas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Eur J Neurosci Asunto de la revista: NEUROLOGIA Año: 2003 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Francia

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links